This research summary has been specifically written for urban land managers or groups who implement actions for biodiversity conservation in urban areas. This report aims to: Develop a national inventory of local actions undertaken for urban biodiversity in Australian cities; Use case studies to showcase the breadth of successful urban biodiversity conservation actions; Summarise common barriers to and enablers of urban biodiversity conservation actions; Document the extent to which Indigenous perspectives are currently considered in urban biodiversity conservation; and Highlight future opportunities to conserve urban biodiversity.