Quantifying the long-term population trajectory of species and the factors affecting these trends is a fundamental part of animal conservation. We undertook a long-term investigation into the occurrence of the greater glider in the wet eucalypt forests of the Central Highlands of Victoria. Using data gathered between 1997 and 2018, we quantified relationships between glider presence and landscape-level variables, including the number of hollow-bearing trees at a site, and changes in the extent of fire and logging in the surrounding landscape over time.