Urban environments provide the opportunity to conserve biodiversity whilst engaging people with urban nature. However, there is currently little guidance for land managers on how to implement conservation actions targeted to urban environments, with no coordinated evidence base documenting opportunities and indicators of success for urban conservation projects. To fill this gap we asked urban land managers from across Australia about their current approaches to urban biodiversity conservation and their perceptions of success. This is the first Australian assessment of its kind and can enable better practice in urban areas across the country.